rabbit polyclonal igg antibody against pparγ  (Cayman Chemical)

Journal: Mediators of Inflammation

Article Title: Activation of Peroxisome Proliferator-Activated Receptor-Gamma by Glitazones Reduces the Expression and Release of Monocyte Chemoattractant Protein-1 in Human Mesothelial Cells

doi: 10.1155/2012/217696

Figure Lengend Snippet: Western Blot analysis of PPAR γ -1 in two different isolates o unstimulated mesothelial cells. Total protein was extracted from unstimulated peritoneal mesothelial cells or human breast carcinoma cells (positive control) and were analysed using the Western Blot technique. The amount of total protein loaded is indicated. Pos. ctrl: positive control; neg. ctrl: negative control.

Article Snippet: The membrane was blocked with 3% skim milk, incubated in a 1 : 1000 dilution of a rabbit polyclonal IgG-antibody against human PPAR γ -1 (SC-7196, Santa Cruz Biotechnology, Heidelberg, Germany) over night, and rinsed with PBS containing 0.1% Tween 20.

Techniques: Western Blot, Positive Control, Negative Control

Journal: Mediators of Inflammation

Article Title: Activation of Peroxisome Proliferator-Activated Receptor-Gamma by Glitazones Reduces the Expression and Release of Monocyte Chemoattractant Protein-1 in Human Mesothelial Cells

doi: 10.1155/2012/217696

Figure Lengend Snippet: (a) PPAR- γ -dependency of the rosiglitazone effect on mesothelial MCP-1 release. MC were preincubated with GW-9662 10 μ M for 24 hours (or with control media) and then incubated with the given conditions for 8 hours. Cell supernatants were examined by ELISA. *indicates a P value < 0.05 ( n = 5). (b) PPAR- γ -dependency of the rosiglitazone effect on mesothelial MCP-1 mRNA expression. MC were preincubated with GW-9662 10 μ M for 24 hours (or with control media) and then incubated with the given conditions for 8 hours. Total RNA was extracted and examined by RT-PCR. The figure shows a representative experiment out of three independent ones.

Article Snippet: The membrane was blocked with 3% skim milk, incubated in a 1 : 1000 dilution of a rabbit polyclonal IgG-antibody against human PPAR γ -1 (SC-7196, Santa Cruz Biotechnology, Heidelberg, Germany) over night, and rinsed with PBS containing 0.1% Tween 20.

Techniques: Control, Incubation, Enzyme-linked Immunosorbent Assay, Expressing, Reverse Transcription Polymerase Chain Reaction